Unveiling Dna Fragment Separation Techniques Through Gel Electrophoresis: A Comprehensive Guide
Gel electrophoresis separates DNA fragments based on their size using an electric field. A gel matrix acts as a sieve, allowing smaller fragments to move faster than larger ones. Negatively charged DNA fragments migrate towards the positive electrode, with smaller fragments traveling further. Ethidium bromide staining allows visualization of the fragments under UV light, forming…